Rough mapping determines the chromosome on which the target mutation genes are by analyzing with the specific primer markers at average 7.5Mb intervals.
To find the gene at a particular site on DNA, Semi-fine mapping and Fine mapping offer more precise mapping by using markers at 2.7Mb intervals or 0.2Mb intervals.
Inplanta Innovations provides the set mapping from Rough through Semi-Fine or the single mapping for Rough, Semi-fine, or Fine. The period taking to analyze one order, for example the order for Rough through Semi-fine mapping, takes only two weeks after we received the samples from our customers.
Prices and analysis duration for Fine Mapping vary with its deals. You can select the specific primer markers and the number of samples. Please contact us for Order made mapping service to which determines the gene domain in one Bac.
The isolated genomic samples taken from at least 20 individuals of F2 population which shows the same phenotype as the target and are the cross-bread of Columbia and Larnsberg (mutation x wild type or wild type x mutation).
Use the Dneasy Plant Kit from QIAGEN for DNA isolation or any other kits that ensure the equal quality. Each sample should be adjusted up to 40ng/ul. Required minimum amount of samples is 50ul.
Please contact us for more details about DNA isolation.
Please note that the samples are sent at customer expense.
Disclaimer
This service is to perform an analysis using SNP-specific markers arranged in 0.2 Mb intervals on the average, and we do not guarantee to “always identify a target gene within the 0.2Mb domain”. The analysis performance depends on the position of the target gene on the chromosome concerned and the number of F2 individuals.
Arabidopsis gene mapping
